Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil. Juliana F. Cargnelutti 1. Eduardo K. Masuda 2.

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E-mail: canavarr uchile. This work reports the detection of canine herpesvirus type 1 CHV-1 in Chile, confirming the strong clinical suspicion of its existence.

A viral isolate, RP5, was obtained from diagnosed clinical cases such as hemorrhagic diseases of puppies.

This viral isolate was inoculated in cell monolayers and produced the typical cytopathic effect of members of the Alphaherpesvirinae subfamily, Herpesviridae family: cellular lysis in a short period of time. This viral isolate demostrated its effect in primary cultures of canine lung and canine kidney, and also in the MDCK cell line.

This effect was lost due to prior incubation of the inoculate with an organic solvent, therefore the adenovirus presence was ruled out. As a specific confirmation of VHC-1 presence, a direct fluorescence test in inoculated cell cultures was carried out using monoclonal antibody anti CHV-1, fluorescein isothiocyanate conjugated VMRD, Inc.

It is of great importance to determine the presence of the virus because the isolation and characterization of the virus will allow to carry out future investigations. This includes the evaluation of the magnitude of infection in the canine population and obtaining information about virus infection repercussion in reproductive places.

In addition it may possible to devise strategies in order to develop an eventual vaccine. Key words : canine herpesvirus, cytopathic effect CPE , direct immunofluorescence. Brevemente, las laminillas con monocapas fueron infectadas con el aislado RP5.

Este resultado preliminar sugiere la presencia de un virus perteneciente a la familia Hespesviridae , agente capaz de provocar in vitro un ECP caracterizado por lisis celular.

Al Dr. Aldo Gaggero B. Herpes simplex virus DNA replication. Detection of canine herpesvirus-1 in a wide range of tissues using the polymerase chain reaction. Identification of a cytopathogenic agent infectious for puppies as a canine herpes virus. Canine herpesvirus as a suspected cause of neonatal mortality in puppies. The immunological relationship between canine herpesvirus and four other herpesvirus. Le virus herpetique canin. A serological study of canine herpesvirus-1 infection in the English dog population.

Canine herpesvirus respiratory infection. Restriction endonuclease analysis of canine herpesviruses isolated in Japan. Identification and nucleotide sequence of the thymidine kinase gene of canine herpesvirus.

Virus Gene Servicios Personalizados Revista. Aceptado: Cytopathic effect observed in canine kidney primary cell cultures infected with RP5 isolate. Inmunofluorescencia directa. Direct immunofluorescence. Negative reaction.

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Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. Canine herpesvirus is an alphaherpesvirus of dogs and there is only one serotype. The virus has been isolated in numerous countries and recent studies in Europe suggest it to be enzootic in the dog population. It is an etiological agent involved in vesicular genital lesions and reproductive disorders such as embryonic resorption, abortion and perinatal mortal- ity. The virus is furthermore associated with respiratory disease kennel cough syndrome. View PDF.


Canine Adenoviruses and Herpesvirus

Canid alphaherpesvirus 1 CaHV-1 , formerly Canine herpesvirus CHV , is a virus of the family Herpesviridae which most importantly causes a fatal hemorrhagic disease in puppies and in wild Canidae less than two to three weeks old. The incubation period of CHV is six to ten days. Low body temperature allows the virus to spread and infect the rest of the body. CHV also causes a necrotizing vasculitis that results in hemorrhage around the blood vessels.

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